This book constitutes the refereed proceedings of the 14th International Conference on Similarity Search and Applications, SISAP 2021, held in Dortmund, Germany, in September/October 2021. The conference was held virtually due to the COVID-19 pandemic.The 23 full papers presented together with 5 short and 3 doctoral symposium papers were carefully reviewed and selected from 50 submissions. The papers are organized in the topical sections named: Similarity Search and Retrieval; Intrinsic Dimensionality; Clustering and Classification; Applications of Similarity Search; Similarity Search in Graph-Structured Data; Doctoral Symposium.